Dr. Marco Cafora, University of Milan, Italy will join the 8th World Congress Targeting Therapy which will be held this June in Berlin.
Topic
Ex vivo pig lung as a new Cystic Fibrosis model for the study of Pseudomonas aeruginosa biofilm infection and phage therapy application
Presentation Summary
Ex vivo pig lung (EVPL) Cystic Fibrosis (CF) model consists of swine bronchial and bronchiolar tissue cubed in an artificial sputum medium. EVPL closely mimics the physicochemical environment of human CF airways, reproducing the metabolic state of the pathogens and enabling the formation of invivo–like biofilm.
Biofilm infections caused by various Pseudomonas aeruginosa strains, including laboratory strains and those isolated from patients with CF, both treated and untreated with ETI.
The ability of various phage preparations (single or cocktail) to eradicate Pseudomonas aeruginosa biofilm has been evaluated both alone and in combination with antimicrobials. Phages exhibited a synergistic effect with antibiotics, leading to a strong reduction in biofilm biomass, disruption of biofilm architecture, and inhibition of biofilm matrix production.
Treatment with single phage preparation (DEV, Schitoviridae) led to near-complete eradication of biofilm formed by Pa clinical isolates derived from PwCF.
Targeting Phage Therapy 2025
June 10-11, 2025 – Berlin, Germany